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Fig. 4. Collaboration of Mer and {alpha}vß5 integrin in phagocytosis of apoptotic cells. (A) HEK-293T cells were transfected with bicistronic pIRES-EGFP (GFP), pIRES-EGFP-ß5 (ß5), pIRES2-EGFP-Mer (Mer) or pIRES-EGFP-ß5 plus pIRES2-EGFP-Mer (ß5+Mer) plasmids. After 48 hours, transfected HEK-293T cells were co-cultured with red-labeled apoptotic T cells. (B) Phagocytosis was assayed 2 hours post co-culture, whereby double positive cells were scored from GFP-expressing cells. Data represent the mean±s.e.m. of triplicate measurements.





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