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Fig. 3. TRIP6 interacts with TRAF2. 293 cells (2x106) were transfected with 5 µg each of plasmids encoding HA-TRIP6 or Flag-tagged TRADD, TRAF2 or IKKß. Cell lysates were immunoprecipitated with anti-Flag antibody ({alpha}-F) or control mouse IgG (C). The immunoprecipitates were analysed by western blot with horseradish-peroxidase (HRP)-conjugated anti-HA antibody. Expression of the transfected proteins in the lysates was comparable, as indicated by western blot analysis with anti-Flag and anti-HA antibodies (bottom).





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