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Fig. 1. An ER retrieval signal does not block targeting to the apicoplast. (A) T. gondii expressing ACP-GFP-HDEL and S9(S)-GFP-HDEL were analyzed by IFA and visualized with anti-GFP, and anti-BiP [S9(S)-GFP-HDEL only], the apicoplast marker quantum red streptavidin and DAPI. A merge of anti GFP or anti BiP, quantum red streptavidin and DAPI is also shown. A merged projection of the marked apicoplasts in the y-z dimension is shown. (B) Anti-GFP immunoblot analysis of protein from ACP-GFP-HDEL (lane 1) and ACP-GFP (lane 2) parasites. t-GFP indicates the species containing the transit peptide and m-GFP the mature form lacking the transit peptide. Bar, 5 µm.