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Fig. 2. Kinetics of cleavage of the apicoplast transit peptide. Intracellular T. gondii expressing ACP-GFP were labeled with 35S amino acids for 1 hour, and chased for 0, 1, 2 and 4.75 hours in control medium. GFP was immunoprecipitated, proteins resolved by SDS-PAGE, and visualized with a phosphorimager. Transit (t-GFP) and mature (m-GFP) forms of ACP-GFP were detected.