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Fig. 5. Extended treatment with BFA does not impede GFP localization to the apicoplast. T. gondii expressing ACP-GFP, or ACP-GFP-HDEL were grown in control medium (con.) with or without 1 µg/ml BFA for 4 hours. Shown are IFAs probed with anti-GFP and the apicoplast marker quantum red streptavidin. A merge of anti-GFP, quantum red streptavidin and DAPI is shown. Bar, 5 µm.





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