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Fig. 10. PLC-
1 association with Src and FAK in TFW-46 and SYF-/- cells. (A) TFW-46 cells were grown in the presence of tetracycline (Tet+) to keep them in the uninduced state. To induce FAK expression, the cells were incubated in tetracycline-free growth medium for the indicated periods of time, then lysed and blotted with anti-FAK and anti-PLC-1 antibody. (B) TFW-46 cells with uninduced (Tet+) and induced (Tet-) FAK expression were allowed to adhere for the indicated times on fibronectin(10 µg ml-1)-coated dishes in serum-free medium. Then, cells were lysed and each lysate was precipitated with anti-Src-kinase antibodies followed by blotting with anti-PLC-1 or anti-Src antibodies. PDL, cells that were allowed to adhere on a poly-D-lysine-coated dish. (C) SYF-/- and Null+ cells were allowed to adhere for the indicated times on fibronectin-coated dishes in serum-free medium. Then, cells were lysed and each lysate was precipitated with anti-FAK antibodies followed by blotting with anti-PLC-1 or anti-FAK antibodies. PDL, cells that were allowed to adhere on a poly-D-lysine-coated dish.
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