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Fig. 3. Influence of PLC-{gamma}1 on spreading and migration behavior. (A) Null and Null+ cells were allowed to adhere to cover glasses coated with the indicated concentration of fibronectin for the indicated times. Actin distribution was then visualized by staining with phalloidin-TRITC. (B) Null (white) and Null+ (gray) cells were applied to fibronectin-coated transwell chambers and allowed to migrate for 3 hours. Subsequently, the chambers were processed to remove all cells from the top and the remaining cells were fixed and stained with crystal violet and counted as migrating cells.





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