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Fig. 4. Influence of PLC-
1 on integrin ß1 clustering, FAK phosphorylation and ERK1,2 phosphorylation following adhesion to fibronectin. (A) Equal numbers of Null and Null+ cells were allowed to adhere for the indicated times to cover glasses coated with 10 µg ml-1 or 2.5 µg ml-1 fibronectin. Integrin ß1 distribution was visualized by staining with anti-integrin-ß1 antibody. (B) Equal numbers of Null and Null+ cells were allowed to adhere to 10 µg ml-1 fibronectin-coated dish for the indicated times. The cells were then lysed and each lysate was blotted with antibodies against phosphorylated or unphosphorylated FAK. (C) Equal numbers of Null and Null+ cells were allowed to adhere to 10 µg ml-1 fibronectin-coated dish for the indicated times. Then cells were lysed and each lysate was blotted with antibodies phosphorylated or unphosphorylated Erk1,2. Cells maintained in suspension (Susp) were used as a control.
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