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Fig. 5. Fibronectin-stimulated tyrosine phosphorylation of PLC- ${gamma}$ 1. (A) Null+ cells were allowed to adhere to fibronectin-coated dishes (10 µg ml^-1) in serum-free medium for the indicated times. The cells were then lysed and each lysate was precipitated with anti-PLC- ${gamma}$ 1 antibody followed by blotting with site-specific antibodies against tyrosine-phosphorylated-PLC- ${gamma}$ 1 or PLC- ${gamma}$ 1 as indicated. (B) Null+ cells expressing wild-type PLC- ${gamma}$ 1 or an N+C-SH2-domain loss-of-function PLC- ${gamma}$ 1 mutant (N+C SH2^-) (Ji et al., 1999) were allowed to adhere to fibronectin (10 µg ml^-1) for the indicated times. The cells were than lysed and the lysate were precipitated with anti-PLC- ${gamma}$ 1 antibody and then blotted with antibody against tyrosine-783-phosphorylated PLC- ${gamma}$ 1 or PLC- ${gamma}$ 1 as indicated. Cells maintained in suspension (Susp) were used as a control for time 0.

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