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Fig. 8. Fibronectin-dependent PLC-1 phosphorylation in SYF^-/- and FAK^-/- cells. (A) SYF^-/- and Null+ cells were allowed to adhere to fibronectin-coated dish (10 µg ml^-1) in serum-free media containing 50 µM sodium pervanadate. The cells were then lysed and each lysate was precipitated with anti-PLC-1 antibodies followed by blotting with site-specific antibodies against tyrosine-783-phosphorylated PLC-1 or PLC-1 as indicated. Cells maintained in suspension (Susp) were used as a control. (B) TFW-46 cells were grown in the presence of tetracycline (Tet+) to maintain an uninduced state. To induce FAK expression, cells were incubated in tetracycline-free growth medium. Cells were allowed to adhere on fibronectin-coated dish (10 µg ml^-1) in serum-free medium containing 50 µM sodium pervanadate. Then, the cells were lysed and each lysate was precipitated with PLC-1 antibodies, followed by blotting with site-specific antibodies against tyrosine-783-phosphorylated PLC-1 or PLC-1 as indicated.

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