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Fig. 9. Fibronectin-induced PLC-{gamma}1 association with Src or FAK. (A) Null+ cells were allowed to adhere for the indicated times on fibronectin-coated (10 µg ml-1) dish in serum-free medium. The cells were then lysed and each lysate was precipitated with anti-Src-kinase antibody followed by blotting with PLC-{gamma}1, FAK or Src antibodies. Whole-cell lysate was loaded as a marker for migration pattern of each protein. (B) Null+ cells expressing wild-type (wt) PLC-{gamma}1 or N+C SH2- PLC-{gamma}1 mutant were allowed to adhere for the indicated times on fibronectin-coated dishes (10 µg ml-1) in serum-free medium. The cells were then lysed and each lysate was precipitated with anti-Src-kinase antibodies followed by blotting with anti-PLC-{gamma}1 or anti-Src antibodies. (C) Null+ cells were allowed to adhere to fibronectin-coated dishes (10 µg ml-1) for the indicated times. Cells were lysed and each lysate was precipitated with anti-FAK antibody followed by blotting with anti-PLC-{gamma}1 or anti-FAK antibodies.





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