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Fig. 1. Gel mobility shift assay (GMSA) of the nuclear and nuclear matrix extracts from mouse liver cells. Nuclear extract (NE, lanes 2-8) and nuclear matrix extract (NM, lanes 9-15) DEAE fractions (5 µg of total protein) were eluted from a DEAE-column by 0.2 M (lanes 2, 3, 9, 10), 0.3 M (lanes 4-6, 11-13) and 0.4 M (lanes 7, 8, 14, 15) NaCl. 1 ng of labelled MiSat fragment was added to the incubation mix. Each probe contained either 50-, 75- or 150-fold weight excess of E. coli DNA as indicated. The GMSA of the mock probe (without proteins added) is shown in lane 1. The fraction of the nuclear matrix eluted by 0.3 M NaCl was chosen for further work. The arrowheads point to the border between two sets of lanes (NE and NM).
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