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Fig. 11. Immunofluorescence of L929 mitotic chromosome spreads. Cells spreads were fixed with methanol:acetic acid (A), with paraformaldehyde (B) and prematurely condensed G2 chromosome preparation (C). P68 is shown in green. Chromosomes were counterstained with DAPI and pseudocoloured in red. Centromeres stained with an antibody against P68 are indicated with white arrowheads; the interchromosome connection is indicated with black arrowheads. Bar, 20 µm.





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