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Fig. 4. JAM-A expression enhances microtubule polymerization. Cells were incubated for 16 hours in either the absence (Control/C) or the presence (Nocodazole/N) of 10 µM nocodazole. In some samples, nocodazole was removed after the 16 hours incubation, and cells incubated for an additional 2 hours (Nocodazole withdrawal/NW). Using the anti-{alpha}-tubulin mAb B-5-1-2, microtubules were analyzed by IF microscopy (A), and NP40-insoluble tubulin was analyzed by western blotting (B). In (B), ZO-1 is shown as a loading control for NP40-insoluble material upon nocodazole withdrawal.





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