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Fig. 5. JAM-A expression stabilizes microtubules. Cells were plated onto fibronectin-coated coverslips and incubated for 90 minutes at 37°C. Then, after the addition of 10 nM taxol (where indicated), cells were further incubated for 30 minutes at 37°C. Finally, cells were incubated at either 37°C or 4°C for an additional 30 minutes, stained with either anti-
-tubulin mAb B-5-1-2 (A) or anti-acetylated--tubulin mAb 6-11B-1 (B).
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