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Fig. 6. JAM-A expression increases the number of focal adhesions. Cells were plated onto fibronectin, incubated for 2 hours, and then stained with either anti-vinculin mAb hVIN-1 (A) or anti-paxillin mAb 349 (B). (C) Individual cells were analyzed for measuring the distribution of the number of vinculin-based focal adhesions per cell; results are derived from three experiments. In (D), cells were plated onto fibronectin for 30 minutes, incubated for an additional 90 minutes in either the absence (Control) or presence of 10 nM taxol, 40 mM lithium and 20 µM PKC{zeta}-PS, and finally stained with anti-vinculin mAb hVIN-1.





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