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Fig. 2. Subcellular localization of Pio and Pot. Distribution of fluorescent fusion proteins in late pupal wing (>70 hours after puparium formation), visualized by confocal microscopy. (A) Owing to the folding of the wing, this confocal section shows the localization of Pot-CFP around the circumference of the stellate apical surface (top) and an optical section through the two attached layer of cells (a cell in each layer is marked with a yellow arrow, with the arrowhead basal) (bottom). (bottom) Pot-CFP is uniformly localized along the apical surface (but not including the wing hair). (B,D) By contrast, at the same stage, a Pio-GFP fusion protein (green) is found in discrete dot-like structures, preferentially at the apical side (D) and near the circumference of the wing epithelial cells as visualized by differential interference contrast (right). (C) Tubulin-GFP shows broader but still discrete points of contact with the apical surface, also enriched at the cell periphery. The outline of one cell is drawn in white in B and C. (E) The basal position of integrin adhesive junctions is shown with integrin-linked kinase (ILK-GFP). (F) Schematic diagram showing the sections of the wing epithelia shown in A-E. Bar, 10 µm (applies to A-E).
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