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Fig. 3. Mitotic spindle orientation is regulated in Drosophila GSCs. (A) Male GSCs expressing a GFP-tagged ${alpha}$ -tubulin were visualized using scanning laser confocal microscopy. Drosophila E-cadherin (red) localizes to the surfaces of hub cells and to the interface between hub cells and GSCs. The mitotic spindle visible in one GSC (arrow) is oriented orthogonally to the apical hub. (B) Immunofluorescence image of a Drosophila germarium. Germ cells are labeled with an antibody to the germ-cell-specific protein Vasa (green). Antibodies to the membrane protein ${alpha}$ -spectrin (red) label the somatic cells within the germarium, as well as the vesiculated, cytoplasmic, ball-shaped structure known as the spectrosome in GSCs (arrow) and cystoblasts.

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