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Fig. 10. EM immunogold localization of nesprin-2 epitopes in C2C12 myoblasts (A) and myotubes (B-F). In myoblasts (not shown) and myotubes, CH3 was present at the NE (black arrows in B) and in punctate nuclear (white arrow in B) localizations. In myoblasts CH3 localized to punctate zones along the plasma membrane (arrows in A) while in myotubes CH3 also decorated cytoplasmic structures, including isolated filaments associated with the plasma membrane (C). N3 was present in cytoplasmic domains in myotubes and decorated the rim of mitochondria (asterisks in D). In myoblasts (not shown) and myotubes, N2 was present at the NE (arrows in E) and in the cytoplasm at the Z-band (long arrows in F) and in a repeating pattern along the length of the myofibrils of the sarcomere (short arrows in F). Bar, 100 nm.





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