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Fig. 3. (A) Northern blots showing nesprin-2 isoform expression in human tissues. Note that a >20 kb band (asterisks) is present in spleen using 5' UTR (1-333 bp)-, 3' UTR (20,673-21,364 bp)- and CH-domain (161-1787 bp)-specific probes confirming that the giant isoform predicted by RT-PCR is transcribed. Highly expressed short transcripts are present in skeletal muscle, heart and pancreas with numerous transcripts of different sizes expressed at low levels in most tissues. (B) Table showing tissue distributions and levels of expression of specific nesprin-2 domains and isoforms as determined by RT-PCR in various human tissue cDNA samples (for details, see Fig. S1 in supplementary material).





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