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Fig. 1. Migfilin localizes to cell-cell junctions in epithelial and endothelial cells. (A-D) Primary newborn human embryonic keratinocytes (A and B), immortalized human keratinocytes (HaCat) (C and D) and primary human microvascular endothelial cells (E and F) were double stained with a mouse monoclonal anti-migfilin antibody (clone 43) (A, C and E) and a rabbit polyclonal anti-ß-catenin antibody (B and F) or anti-FAK antibody (D). The images were recorded using a Leica DM R fluorescence microscope equipped with a Hamamatsu ORCA-ER digital camera. The cell-cell and cell-ECM adhesions are indicated by arrows and arrowheads, respectively. Note that a fraction of migfilin was co-clustered with ß-catenin at cell-cell junctions (arrows, C) that lack FAK (D). Migfilin was also detected at FAK-rich cell-ECM adhesions (arrowheads, C and D). Bars in A, C and E, 8 µm.
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