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Fig. 11. Depletion of migfilin compromises the organization of adherens junctions. Human HT-1080 cells that were transfected with the migfilin siRNA (lane 2) or the control small RNA (lane 1) were analyzed by western blotting with monoclonal anti-migfilin antibody (clone 43) (A) or an anti-cadherin antibody (C). Equal loading was confirmed by re-probing the migfilin membrane (A) with a monoclonal anti-actin antibody (B). (D-I) HT-1080 cells transfected with the control RNA (D, F and H) or the migfilin siRNA (E, G and I) were double stained with the mouse monoclonal anti-migfilin antibody (clone 43) (F and G) and Rhodamine-conjugated phalloidin (H and I). The mouse anti-migfilin primary antibody was detected with a FITC-conjugated anti-mouse IgG secondary antibody. (J-O) HT-1080 cells transfected with the control RNA (J, L and N) or the migfilin siRNA (K, M and O) were double stained with a rabbit polyclonal anti-ß-catenin antibody (L and M) and a mouse monoclonal anti-cadherin antibody (clone CH-19) (N and O). The DIC (D, E, J and K) and immunofluorescent (F-I and L-O) images were recorded as described in Fig. 10. Bar, 8 µm.
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