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Fig. 2. Immunofluorescent confocal microscopic analysis of cell-cell adhesion localization of migfilin. Human immortalized keratinocytes (HaCat cells) were double stained with monoclonal anti-migfilin antibody (clone 43) (A and D) and a rabbit polyclonal anti-ß-catenin antibody (B and E) observed under an Olympus Fluoview BX61 confocal microscope. The x-z sections at the lateral (A and B) and basal (D and E) levels were shown. (C,F) Images merged from the migfilin (green) and ß-catenin (red) staining at the lateral and basal levels, respectively. The arrow in C indicates the position of the x-z cross section (G). Bar, 8 µm.





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