QUICK SEARCH:   [advanced] Author: Keyword(s):
Home     Help     Feedback     Subscriptions     Archive     Search     Table of Contents

Help viewing high resolution images Return to article

Fig. 7. The C-terminal LIM domains, but not the N-terminal filamin-binding domain or the central proline-rich domain, mediate migfilin localization to adherens junctions. (A-D) The central proline-rich domain is not required for migfilin localization to cell-cell junctions. HaCat cells were transfected with expression vectors encoding GFP-migfilin (A and B) or GFP-migfilin(s) (C and D). The cells were stained with the rabbit anti-ß-catenin primary antibody and Rhodamine Red^TM-conjugated anti-rabbit IgG secondary antibody, and were observed under a Leica DM R fluorescence microscope equipped with GFP (A and C) or rhodamine (B and D) filters. (E-J) The C-terminal LIM domains, but not the N-terminal filamin-binding domain, are sufficient for mediating migfilin localization to adherens junctions. HaCat cells that were transiently transfected with expression vectors encoding GFP-PR-LIM1-3 (E and F), GFP-LIM1-3 (G and H) or GFP-N-ter (I and J) were stained with the anti-ß-catenin antibody or phalloidin as indicated. Bar, 8 µm.

Return to article