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Fig. 8. Effect of cysteine point mutations on the adhesive capacity of monomeric N-cadherin-Fc. (a) The assay was conducted in HBSS + 2%FCS (oxidising conditions). (b) Reducing conditions were established by adding 10 mM DTT. Approximately 65% of wild-type cells adhered to the plate in both assays. Reduction restored adhesive capacity of the double mutants D1C,R25C and D1C,D27C.





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