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Fig. 9. Expression of cell surface N-cadherin by K562 transfectants and by DX3 melanoma cells. (a) Stable K562 transfectants were stained with mAb NCD-2 to chicken N-cadherin. The four panels show comparable levels of expression. (b) Untransfected K562 cells were tested with mAb 8C11 to human N-cadherin and the first panel verifies that these cells do not naturally express N-cadherin. The final panel shows DX3 cells stained with 8C11, showing strong expression of human N-cadherin. (c) The molecular size of cellular N-cadherin from K562 transfectants (Wt) is compared with that of monomeric N-cadherin Fc fusion protein (Wt) bearing the mutations F405A,Y407A in the Fc region to prevent dimerization. N-cadherin extracted from normal myoblast cells is also shown for comparison. The gel was run under non-reducing conditions and blotting was conducted using a mixture of pan cadherin antibody to the cytoplasmic domain and anti-Fc.
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