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Fig. 1. Exit from mitosis 15 after failure of sister chromatid separation in pim mutant embryos. Time-lapse in vivo imaging was used for the analysis of progression through the fifteenth round of mitosis during Drosophila embryogenesis in pim1 mutant (pim1) and pim+ (pim+) sibling embryos expressing red fluorescent histone H2AvD-mRFP and a green fluorescent microtubule-binding protein. Selected frames showing microtubule distribution (top) or merged color images (bottom) are shown. Numbers below the frames indicate the time in minutes relative to the last metaphase frame, which was set to zero. The appearance of prominent interpolar microtubules characteristic of anaphase is indicated by arrowheads. Central spindle and midbody during telophase are indicated by arrows. Bar in upper right frame, 5 µm.





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