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Fig. 4. Centrosome numbers in pim mutants. Embryos were labeled at the stage of mitosis 16 (A,B,D) or mitosis 15 (C) with a DNA stain (blue), with anti-phospho-histone H3 (PH3, green) to identify mitotic cells, with anti-Bazooka (Baz, green) to define cell boundaries, and with anti-{gamma}-tubulin (red, {gamma}Tub) to reveal centrosomes. Maximal projections of representative cells from a pim1 mutant (B, pim1), a pbl mutant (C, pbl), a pim1pbl double mutant (D, pim1 pbl) and a sibling pim+ embryo (A, pim+) are shown, indicating that centrosomes are duplicated in pim1 mutants during cycle 16 when cytokinesis during mitosis 15 is inhibited by homozygosity for pbl (see text for further explanations). Bar, 3 µm.





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