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Fig. 4. Effects of retrovirally transfected apo J against UVB-induced SIPS. (A) Overexpression of human apo J after retrovirally-mediated overexpression in skin HDFs. (a) Overexpression at mRNA level of both native and tagged (V5/His) apo J compared to empty PLXSN vector. Real time RT-PCR was used to detect apo J mRNA. (b) Overexpression of protein level of both native and tagged (V5/His) apo J compared to empty pLXSN vector (western blotting). (B-D) Effects of retrovirally transfected apo J against SIPS induced by 10 subcytotoxic exposures to 250 mJ/cm² UVB in skin HDFs. SA ß-gal activity (B), incorporation of [³H]thymidine (C) and relative steady-state mRNA level of fibronectin (D) were studied as described in Materials and Methods. Skin HDFs transfected with the empty pLXSN vector, with pLXSN/apo J vector or pLXSN/apo J-V5/His vector were exposed (gray columns) or not (white columns) to UVB. Results are given as mean ± s.d. of three independent experiments except (D) where pools of RNA were obtained from extracts of RNA from three independent experiments. Statistical analysis was carried out with the Student's t-test. ns, non-significant (P>0.05); ^*, 0.05>P>0.01; ^**, 0.01>P>0.001; ^***, P<0.001.

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