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Fig. 7. A series of 10 exposures of skin HDFs to UVB at 250 mJ/cm2 increases sharply the abundance of both latent and active forms of TGF-ß1. Skin HDFs were given a series of 10 exposures to UVB at 250 mJ/cm2 and seeded on glass cover slides at 16, 40 or 64 h after the last stress. Control cells were submitted to the same culture conditions but not exposed to UVB. At 24 hours after seeding, the activation of TGF-ß1 was investigated by immunofluorescence using antibodies recognizing the latent form of TGF-ß1 (LAP) (green; left images) and the active TGF-ß1 (red; middle images) using semi-quantitative confocal microscopy. (Right images) Superimposition of phase contrast and fluorescence with green and red emission.





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