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Fig. 2. Co-localization of epiplakin with keratin and vimentin IF networks. (A) Triple-label immunostaining in HeLa cells with antibodies to epiplakin (epip), vimentin (vim) and keratin (kerat). The co-localization of epiplakin and vimentin appears yellow and is indicated by arrowheads. Co-localization of the three proteins appears white (arrows). Bars, 20 µm. (B) Immunostaining of epiplakin (epip) and keratin 14 (K14) in HaCaT cells. The intense epiplakin staining is indicated by arrowheads (a). Boxed area in c is shown at higher magnification in d-f. The branch structure of the network is indicated by arrows. Bars, 10 µm. (C) Immunostaining of keratinocytes maintained in low calcium (a-c) and high calcium medium (one day; d-f). Cells were fixed and stained with antibody of epiplakin (epip) and keratin 5 (K5). Arrows indicate co-localization. Bars, 20 µm.





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