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Fig. 4. Overlay binding assay of epiplakin fragments with vimentin, keratins and assembled IFs. (A) Schematic illustration of human epiplakin 9 [according to the nomenclature of Fujiwara et al. (Fujiwara et al., 2001)]. (B) Dot-blot binding assay of recombinant purified monomeric or polymerized IF proteins with expressed epiplakin fragments (right: schematic representation of the GST-fused epiplakin constructs). The indicated recombinant proteins were spotted in duplicate onto three membranes as described in Materials and Methods. The expressed GST-EpiL12B13 protein (lane 1) served as a positive control for GST antibody. Protein loading was monitored by Amido Black staining. The experiments were repeated in at least three times and one representative set of data is shown.





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