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Fig. 6. The knock down of epiplakin in epidermal cells. (A) Immunofluorescent staining of epiplakin in NHEK transiently transfected with the indicated RNA duplex and maintained in either low calcium (NHEK-Low; a,b) or high calcium (NHEK-Hi; c,d) medium for 2 days, fixed and stained with anti-epiplakin antibody (red). Nuclei were stained with TO-PRO 3 (blue). Bars, 20 µm. (B) Immunofluorescent staining in HaCaT cells transiently transfected with 50 nM of siRNA-SC (a) or siRNA-KD (b,c) were stained with anti-epiplakin (epip; a,b) and anti-keratin (kerat; c). b and c are from the same field. Bars, 20 µm.





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