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Fig. 7. The effects of stable knock down of epiplakin on cytoskeletal structures. (A) Immunoblotting analysis of HeLa stable cell lines. Equal amounts of cellular extracts from two stable cell lines of pSilent/Epi-SC (lane 1, 2) or pSilent/Epi-KD (lane 3, 4) were fractionated on a SDS-NuPAGE gel and probed with anti-epiplakin antibody. The molecular mass of nebulin (850 kDa) is marked. (B) Immunofluorescent staining of the stable HeLa cell lines. The stable epiplakin knockdown cells were stained with the antibodies to epiplakin (epip), keratin (kerat), and also with either anti-ß-actin (actin) antibody (a-c), or anti-ß-tubulin (tub) antibody (d-f). The control (g-i) was stained with antibodies of epiplakin (epip), keratin (kerat) and vimentin (vim). a-c, d-f and g-i are from the same field. Bars, 20 µm.





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