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Fig. 8. The effects of epiplakin linker and B domain on the IF network in the absence of epiplakin. (A) Immunofluorescence analysis of HeLa cells transiently transfected with siRNA-KD together with pEGFP-B13 (a-c), pEGFP-L12 (d-f) or pEGFP-L12B13 (g-i). Cells were fixed and double-stained with anti-vimentin (vim; b,e,h) and anti-keratin (kerat; c,f,i) antibodies. a, d and g show the green fluorescence signal of EGFP. Panels a-c, d-f and g-i are from the same field. Bars, 20 µm. (B) Immunofluorescence analysis of stable epiplakin knockdown HeLa cell lines. Cells were transiently transfected with pEGFP-L12B13 and were double-stained with anti-epiplakin (epip; b,e), anti-keratin (kerat; c) or anti-vimentin (vim; f) antibodies. a and d show the green fluorescence signal of EGFP. a-c and d-f are from the same field. Bars, 20 µm.





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