JCS01657 Supplementary Material

Reference

Macara, I. G. (2001) Transport into and out of the nucleus. Microbiol. Mol. Biol. Rev. 65, 570-594.

Files in this Data Supplement:

• Supplemental Figure 1 -

  Fig. S1. FOXM1 genomic organization and isoforms. The genomic organization of the muse and human FOXM1 loci was predicted based on sequence analysis. Shown below the genome organization are schematic diagrams of the three alternatively spliced FOXM1 isoforms, FOXM1a, FOXM1b and FOXM1c, previously identified. Exon VIIa is uniquely present in FOXM1a. However, exon VIIa could not be identified in the mouse as exons VII and VIII are fused to form a single exon. Extensive search of human EST databases using exon VIIa also failed to identify FOXM1-related matches. These analyses raise doubt about the existence of FOXM1a and support FOXM1b and FOXM1c as the major FOXM1 isoforms.

• Supplemental Figure2 -

  Fig. S2. A putative NLS is present in exon VI. Within exon VI (amino acids 341-422), the sequence RRNMTIKTELPLGARRKMK matches the consensus sequence of a typical bipartite NLS: BBX_10-12(B₃X₂) (Macara, 2001) (B, basic amino acid; X, any amino acid; letters in bracket can be in any order). This sequence is highly conserved as indicated by an alignment of exon VI sequences from the human and mouse; the only change is a substitution of the first arginine (R) in the human sequence by histidine (H) in the mouse sequence.