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Fig. 4. FOXM1c is the predominant isoform expressed in BJ1, NIH 3T3 and various mouse tissues. (A) RT-PCR assays were designed using human and mouse primers, which flank exon Va, to assess the expression of FOXM1b and FOXM1c transcripts. FOXM1b and FOXM1c transcripts generate PCR fragments of 323 and 368 base pairs, respectively. (B-D) RT-PCR analysis. As positive controls, cDNAs encoding human FOXM1b and FOXM1c, and human and mouse testis cDNA libraries, were PCR-amplified to generate the FOXM1b and FOXM1c bands. GAPDH transcripts were also PCR-amplified in the mouse tissue samples to control for possible loading differences. mRNA of asynchronized and synchronized BJ1 cells [0 hour (G1), 4.5 hours (S), 9 hours (G2) after aphidicolin release] (B) and asynchronized, L-mimosine-arrested (G1/S) and etoposide-arrested (G2) NIH 3T3 cells (C) were extracted for RT-PCR analysis. Intestine (I), heart (H), liver (Li) and lung (Lu) of day 1 neonates, and pancreas (P) and thymus (Th) of adult mice, were harvested for RT-PCR analysis and compared to that of testis (Te) cDNA library (D).





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