|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 8. Arp2/3 complex binding is required for cortactin to interact with dynamin 2. (A) Expression of cortactin fusion proteins was analyzed by immunoblotting whole cell lysates with cortactin antibody. (B) 2x105 Cells expressing cortactin-GFP fusions were seeded on a 12-well plate, grown in a serum-free medium at 37°C for 30 minutes and incubated with biotin-transferrin for 40 minutes on ice to allow sufficient binding. Endocytosis was initiated by incubation at 37°C for the times as indicated and terminated by placing on ice. The treated cells were then subjected to ELISA-based biotin-transferrin uptake assay as described in Materials and Methods.
|
