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Fig. 3. Ectopic mTERT expression affects cell growth under physiological conditions. (A) Growth curve of pcDNA-transfected and mTERT-expressing ES cells. Representative experiment is shown. Data represent means±s.d. from one of the experiments in duplicate. (*) represents P<0.05 in a one-tailed unpaired Student's t test. (B) Proliferating cells were identified by labelling with BrdU for 30 minutes and followed by staining with FITC-conjugated anti-BrdU antibody. Representative flow-cytometry results are shown. The gated area represents the BrdU+ proliferating cells. (C) Basal cell death was determined in pcDNA-transfected and mTERT-overexpressing ES cells by PI exclusion. Data represent means±s.d. from one of the experiments in triplicate. (*) represents P<0.05 in a one-tailed unpaired Student's t test. (D) Undifferentiated ES cells were induced to differentiate with 0.3 µM retinoic acid for 3 days and the cumulative cell death over the course of differentiation was determined as described in Fig. 3C. Data represent means±s.d. from one of the three independent experiments in duplicate. (*) indicates P<0.05 in a one-tailed unpaired Student's t test.





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