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Fig. 8. Effect of p53 inhibition and deficiency on mTERT-mediated resistance to cell death. (A) Pifithrin 
(PFT) (10 µM) was added to cells 24 hours before cisplatin treatment in order to inhibit p53. Cell death was then determined upon treatment with various doses of cisplatin in the presence of PFT by PI exclusion. Data represent means±s.d. from one of the experiments in duplicate. (B) Expression of p53 in undifferentiated CCE ES cells (p53+/+) and P1.1 ES cells (p53–/–) was determined by RT-PCR. 3 µg RNA was extracted from undifferentiated ES cells and reverse transcribed into cDNA for PCR. (C) Expression of mTERT in the indicated undifferentiated ES cells was determined by RT-PCR as described above. (D) Undifferentiated CCE ES cells (p53+/+) and P1.1 ES cells (p53–/–) were treated with various doses of doxorubicin for 12 hours, and cell death was determined by PI exclusion. Data represent means±s.d. from one of the experiments in duplicate.
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