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Fig. 6. Effects of ARL1 depletion on endocytosis. Receptor-mediated endocytosis was analysed by monitoring the uptake of FITC-labelled ConA in BSF line Bp2T7/ARL1 grown in the absence (-Tet) and presence (+Tet) of tetracycline for 24 and 36 hours. All cells are co-stained with DAPI (blue). (A) In cells incubated for 30 minutes at 12°C, ConA (green) is present in the endosomes. (B) In cells incubated for 30 minutes at 37°C, all ConA has reached the lysosome, except in parasites induced for 36 hours, which still contain a significant quantity of ConA in the endosomes. (C) Colocalisation of FITC-ConA (green) with the lysosomal marker p67 (red) in cells as in B. Bar, 10 µm.





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