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Fig. 1. BBS6 is a highly divergent eukaryotic Group II chaperonin related to CCT. (A) Maximum likelihood phylogenetic tree constructed from an alignment containing 5 BBS6 sequences, 11 archaeal chaperonins and 32 eukaryotic CCTs (four from each of the eight different CCT subunits). The BBS6 sequences are highlighted, and PROML bootstrap support values are provided for significant nodes on the tree where they are >50%. The BBS6 proteins show a weakly supported but consistently observed relationship with the {alpha} subunit of CCT, suggesting that the gene encoding BBS6 is a divergent, duplicated CCT{alpha} subunit. The scale bar indicates the estimated number of amino acid substitutions per site. Abbreviations: Cren; Crenarchaeotes, Eur; Euryarchaeotes. (B) Amino acid identities shared between Homo sapiens, Mus musculus, Danio rerio and Ciona intestinalis BBS6 and CCT{alpha} protein sequences. (C) BBS6 proteins share signature sequences that differ from other chaperonins. Alignments of two sequences from human BBS6 (HsBBS6), Danio BBS6 (DrBBS6) and Ciona BBS6 (CiBBS6) are compared to a consensus sequence derived from CCT and archaeal chaperonins (see Fig. S1 in supplementary material). Positions known to interact with the different regions of the ATP moiety (Ditzel et al., 1998) are indicated with inverted triangles under the alignments.





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