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Fig. 3. BBS6 is a centrosomal protein that is also found at the midbody during cytokinesis. (A) Two discrete perinuclear signals are observed in IMCD3 cells by immunocytochemistry using an affinity-purified anti-BBS6 polyclonal antibody. (B) Immunolocalization studies of endogenous BBS6 (top panels) and transiently expressed myc-tagged, and GFP-tagged BBS6 (middle and bottom panels, respectively) show that BBS6 colocalizes with the centrosomal marker, 
-tubulin. The transfected cells were fixed and costained with antibodies to myc and -tubulin, and GFP-BBS6 signal was detected by direct GFP fluorescence (middle and bottom panels). Left, middle and right panels, respectively, show BBS6 (green), -tubulin (red) and a merge of the two signals (yellow indicates overlap). (C) Endogenous BBS6 is found at both basal bodies (mother centrioles, displaying a primary cilium) and daughter centrioles. BBS6 is stained green and cilia are stained red with anti-acetylated-tubulin antibodies. (D) BBS6 remains associated with centrosomes throughout the cell cycle. Synchronized IMCD3 cells were co-stained with antibodies to BBS6 (green) and -tubulin (red); yellow indicates overlapping signals. (E) BBS6 is found at the centrosomes (indicated by arrowheads) and midbody region (indicated by an arrow) during telophase, as judged by its colocalization with -tubulin.
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