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Fig. 4. Locus decondensation in lines E and F. After hybridization with pHMW-1Ax1 and pHMW-1Dx5 to detect the transgene loci the fluorescent area per optical section was measured in 79 and 18 randomly selected nuclei of lines E and F, respectively, at 6 dpa and in 59 and 18 nuclei of lines E and F, respectively, at 14 dpa. The total number of voxels in the fluorescent region per nucleus was multiplied by the voxel volume and the resulting fluorescent volume is shown on the y-axis.





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