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Fig. 7. Loss of 
6ß4 integrin does not affect proliferation/survival of basal keratinocytes when cells are adhered to the BM. (A-F) Cryosections of skin from newborn ß4flox/flox; K14-Cre mice, injected intraperitoneally with BrdU for 1 hour (A-C) or assayed by TUNEL for the presence of apoptotic cells (D,F) that were subsequently processed for indirect immunofluorescence and visualized by confocal microscopy. Primary antibodies are against proteins indicated, and colors are coded according to FITC or Texas Red secondary antibodies. In all panels, epidermis is at the top and dermis at the bottom. (G) Quantitative analysis of cell proliferation in the interfollicular regions of epidermis that lack or contain the ß4 integrin. (H) The NMK-1(+) and (–) cell populations do not form colonies in semi-solid medium (left panels) and show the same growth capacities at low density (right panel). (I) Growth curves indicate that the NMK-1(+) and (–) cell populations have the same growth characteristics under the various conditions tested. (J) Representative DNA histograms of exponentially growing NMK-1(+) and (–) cells. (K) Immunofluorescent detection and quantification of BrdU incorporation in NMK-1(+) and (–) cells. (L) Erk1/2 phosphorylation in growth-factor-stimulated NMK-1(+) and (–) cells. Cells were cultured for 16 hours in keratinocyte serum-free medium after which EGF and pituitary gland extract were added for the time periods indicated. Lysates were blotted and probed with antibodies against ß4, phospho-Erk and total Erk as control.
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