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Fig. 5. Proteasome inhibition increases the phosphorylation of MAP kinase p42/p44. HUVECs and ECV-304 cells were serum-starved overnight and treated with MG-262 (600 nM). Phosphorylation of MAP kinase p42/p44 was analyzed by immunoblot after 0, 90 and 180 minutes of treatment (upper panels). Corresponding amounts of total p42 are shown in lower panels.





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