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Fig. 9. The association of TACE with proteasome inhibitors increases the cell susceptibility to the proapoptotic effect of TNF-{alpha}. ECV-304 cells were incubated overnight in the absence of serum with Ro 32-7315 (5 mM) and/or MG-262 (30 nM) in presence of TNF-{alpha} (60 U/ml) as indicated. Surface binding of annexin V-FITC and propidium iodide labeling were measured by flow cytometry. Apoptotic cells are in square `a', necrotic cells in square `n' and secondary necrotic cells in square `a/n'. The percentage of apoptotic cells (a and a/n) is indicated. Ro, Ro32-7315. Experiments were performed in triplicate and repeated twice.





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