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Fig. 5. Colocalization of trichoplein and K8/18 filaments in HeLa cells. (A) HeLa cells were double-stained with trichoplein and K18 antibodies. The enlargement of the area indicated by an arrowhead and merged images are also shown. (B) Electron microscopic immunolocalization of trichoplein in HeLa cells. Antibody binding sites were detected predominantly in keratin-rich regions (left). At high magnification (right), staining is obvious on the bundle of keratin filaments (arrow). (C) HeLa cells in mitosis were double-stained for K18 and trichoplein. Bar, 10 µm (A,C); 500 nm (B, left); 50 nm (B, right).





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