|
|
|
|
|
|
|
|
|||||
| Home Help Feedback Subscriptions Archive Search Table of Contents | |||||
|
Fig. 8. Distribution of trichoplein in the absorptive cells of the small intestine. Frozen sections of the small intestine were double stained with trichoplein antibody and the markers. Then, they were analyzed by laser-scanning confocal microscopy. (A) Double-stained absorptive cells with K8/18 and trichoplein antibodies. In the lower magnification of a nearly vertical view (a-c) and in the higher magnification of a horizontal view (d-f), trichoplein was associated with K8/18 in the apical region (terminal web). Panel e and arrowheads in b show trichoplein concentrating at the cell-cell border. (B) Double-stained absorptive cells with desmoplakin and trichoplein antibodies. In the lower magnification image of a nearly vertical view (g-i) and the higher magnification of a horizontal view (j-l), trichoplein is closely associated with desmoplakin at the cell-cell border. (C) Higher magnification of the vertical sectional views of the absorptive cells double stained with trichoplein antibody and K8/18 antibody or antibodies for the markers of junctional complex (ZO-1, ß-catenin or desmoplakin 1&2). Trichoplein is located close to K8/18-positive signals of higher density in the apical region (m-o). At the cell-cell border, trichoplein closely associates with desmoplakin (p-r), and does not always merge with ZO-1 (s-u) or ß-catenin (v-x). These observations are similar to those of Caco-2 cells in Fig. 6. Bar, 10 µm.
|
