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Fig. 4. Analysis of band 3 clustering. (A) Example of a line profile of fluorescence intensity drawn at the periphery of the center section of a deconvolved erythrocyte (arrow). Bar, 10 µm. (B) A graphical representation of the profile shown in A. 1 pixel=45 nm. (C) Power spectrum of the frequency profile of measured fluorescence fluctuations shown in B plotted as the inverse of pixel distance (f). (D) Double logarithmic plots of power spectra show linear relationship between frequency and normalized power. Multiple data sets for a developmental stage in AA erythrocytes and their linear regression lines are shown. (E) A comparison of regression slope values between AA and CC erythrocytes as a function of parasite developmental stage. These slopes demonstrate that CC erythrocytes have higher degrees and rates of clustering than AA erythrocytes throughout the intracellular parasite cycle. (F) Autocorrelation analyses of measured fluorescence intensity profiles of representative uninfected and parasitized AA and CC erythrocytes. Only the first 1 µm of the intensity profile is shown for clarity. Autocorrelation values were normalized against the value at C₀. The first decay function of the data was fitted (solid lines) using a first-order exponential function to estimate characteristic decay lengths. The slower decay values shown by both uninfected and parasitized CC cells and parasitized AA erythrocytes represent higher degrees of band 3 clustering.

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